Fluorescent D-amino acids (FDAAs) were developed in collaboration with Michael VanNieuwenhze’s laboratory.
Obtaining FDAAs:
***send requests to m_vannieuwenhze@baylor.edu
Uses and properties of FDAAs
Fluorescent D-amino acids (FDAAs) enable the fluorescent labeling of areas of peptidoglycan synthesis in most bacterial species. FDAAs are covalently incorporated into peptidoglycan through the activity of peptidoglycan synthases, indicating the formation and remodeling of peptidoglycan in situ.
The main publications describing FDAAs and their use are:
- In Situ probing of newly synthesized peptidoglycan in live bacteria with fluorescent D-amino acids.
Kuru E, Hughes HV, Brown PJ, Hall E, Tekkam S, Cava F, de Pedro MA, Brun YV, VanNieuwenhze MS.
Angew Chem Int Ed Engl. 2012 Dec 7;51(50):12519-23. doi: 10.1002/anie.201206749. - Synthesis of fluorescent D-amino acids and their use for probing peptidoglycan synthesis and bacterial growth in situ.
Kuru E, Tekkam S, Hall E, Brun YV, Van Nieuwenhze MS.
Nat Protoc. 2015 Jan;10(1):33-52. doi: 10.1038/nprot.2014.197. - Full color palette of fluorescent d-amino acids for in situ labeling of bacterial cell walls.
Hsu YP, Rittichier J, Kuru E, Yablonowski J, Pasciak E, Tekkam S, Hall E, Murphy B, Lee TK, Garner EC, Huang KC, Brun YV, VanNieuwenhze MS.
Chem Sci. 2017 Sep 1;8(9):6313-6321. doi: 10.1039/c7sc01800b.
FDAA structures and properties
